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2021-07-20
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Introduction to Microscope Slide
Introduction to Microscope Slide
1. Bibliography of microscope slideRichard Cote, Saul Suster, Lawrence Weiss, Noel Weidner (Editor). Modern Surgical Pathology (2 Volume Set). London: W B Saunders. ISBN0-7216-7253-1.CS1 maint: multiple names: authors list (link) CS1 maint: extra text: authors list (link).mw-parser-output cite.citationfont-style:inherit.mw-parser-output .citation qquotes:"""""""'""'".mw-parser-output .id-lock-free a,.mw-parser-output .citation .cs1-lock-free abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/6/65/Lock-green.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .id-lock-limited a,.mw-parser-output .id-lock-registration a,.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/d/d6/Lock-gray-alt-2.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .id-lock-subscription a,.mw-parser-output .citation .cs1-lock-subscription abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Lock-red-alt-2.svg/9px-Lock-red-alt-2.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/a/aa/Lock-red-alt-2.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registrationcolor:#555.mw-parser-output .cs1-subscription span,.mw-parser-output .cs1-registration spanborder-bottom:1px dotted;cursor:help.mw-parser-output .cs1-ws-icon abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Wikisource-logo.svg/12px-Wikisource-logo.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/4/4c/Wikisource-logo.svg");background-repeat:no-repeat;background-size:12px;background-position:right .1em center.mw-parser-output code.cs1-codecolor:inherit;background:inherit;border:inherit;padding:inherit.mw-parser-output .cs1-hidden-errordisplay:none;font-size:100%.mw-parser-output .cs1-visible-errorfont-size:100%.mw-parser-output .cs1-maintdisplay:none;color:#33aa33;margin-left:0.3em.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration,.mw-parser-output .cs1-formatfont-size:95%.mw-parser-output .cs1-kern-left,.mw-parser-output .cs1-kern-wl-leftpadding-left:0.2em.mw-parser-output .cs1-kern-right,.mw-parser-output .cs1-kern-wl-rightpadding-right:0.2em.mw-parser-output .citation .mw-selflinkfont-weight:inherit------2. Function of microscope slideBefore HM Government wind-up led by minister James Brokenshire, the FSS was the market leader in the supply of forensic science services to police forces in England and Wales, as well as being a source of training, consultancy and scientific support. The FSS originally set up and maintained the UK National DNA Database, but it is now run by the National Policing Improvement Agency (NPIA).The FSS suffered damage to its reputation following the failure to recover blood stains from a shoe in the murder of Damilola Taylor. Further damage occurred when the FSS failed to use the most up-to-date techniques for extracting DNA samples in cases between 2000 and 2005. This led the Association of Chief Police Officers (ACPO) to advise all police forces in England and Wales to review cases where samples had failed to give a DNA profile.TechnologiesThe FSS's innovative and highly sensitive DNA profiling technique called LCN (low copy number) was used in convicting Antoni Imiela (the M25 rapist) and Ronald Castree (for the murder of Lesley Molseed in 1975), but was questioned during the 2007 trial of a suspect in the Omagh bombing. However, a review by the CPS found that "the CPS has not seen anything to suggest that any current problems exist with LCN. Accordingly we conclude that LCN DNA analysis provided by the FSS should remain available as potentially admissible evidence". In addition, other Police Forces around the world are reviewing cases where LCN DNA profiling resulted in the successful prosecution of suspects. Included in this are several high-profile international cases including the murder of Swedish Foreign Minister Anna Lindh by Mijailo Mijailovic and in Australia, the murder of a backpacker Peter Falconio by Bradley John Murdoch and trial of Bradley Robert Edwards for the Claremont serial killings.In later years the FSS drew on internal expertise and key international experts to become a pioneer in forensic software and technology, notably DNA interpretation, databasing, and electronic forensics.------3. Background of microscope slide24 March commemorates the day in 1882 when Dr Robert Koch astounded the scientific community by announcing to a small group of scientists at the University of Berlin's Institute of Hygiene that he had discovered the cause of tuberculosis, the TB bacillus. According to Koch's colleague, Paul Ehrlich, "At this memorable session, Koch appeared before the public with an announcement which marked a turning-point in the story of a virulent human infectious disease. In clear, simple words Koch explained the aetiology of tuberculosis with convincing force, presenting many of his microscope slides and other pieces of evidence." At the time of Koch's announcement in Berlin, TB was raging through Europe and the Americas, causing the death of one out of every seven people. Koch's discovery opened the way toward diagnosing and curing tuberculosis------4. Botanical illustrator of microscope slideA botanical illustrator is a person who paints, sketches or otherwise illustrates botanical subjects. Typical illustrations are in watercolour, but may also be in oils, ink or pencil, or a combination of these. The image may be life size or not, the scale is often shown, and may show the habit and habitat of the plant, the upper and reverse sides of leaves, and details of flowers, bud, seed and root system.Botanical illustration is sometimes used as a type for attribution of a botanical name to a taxon. The inability of botanists to conserve certain dried specimens, or restrictions on safe transport, have meant illustrations have been nominated as the type for some names. Many minute plants, which may only be viewed under a microscope, are often identified by an illustration to overcome the difficulties in using slide mounted specimens. The standards for this are by international agreement (Art 37.5 of the Vienna Code, 2006)...------5. Specimens of microscope slideThere are two major types of specimens submitted for surgical pathology analysis: biopsies and surgical resections.A biopsy is a small piece of tissue removed primarily for the purposes of surgical pathology analysis, most often in order to render a definitive diagnosis. Types of biopsies include core biopsies, which are obtained through the use of large-bore needles, sometimes under the guidance of radiological techniques such as ultrasound, CT scan, or magnetic resonance imaging. Core biopsies, which preserve tissue architecture, should not be confused with fine-needle aspiration specimens, which are analyzed using cytopathology techniques. Incisional biopsies are obtained through diagnostic surgical procedures that remove part of a suspicious lesion, whereas excisional biopsies remove the entire lesion and are similar to therapeutic surgical resections. Excisional biopsies of skin lesions and gastrointestinal polyps are very common. The pathologist's interpretation of a biopsy is critical to establishing the diagnosis of a benign or malignant tumor, and can differentiate between different types and grades of cancer, as well as determining the activity of specific molecular pathways in the tumor. This information is important for estimating the patient's prognosis and for choosing the best treatment to administer. Biopsies are also used to diagnose diseases other than cancer, including inflammatory, infectious, or idiopathic diseases of the skin and gastrointestinal tract, to name only a few.Surgical resection specimens are obtained by the therapeutic surgical removal of an entire diseased area or organ (and occasionally multiple organs). These procedures are often intended as definitive surgical treatment of a disease in which the diagnosis is already known or strongly suspected. However, pathological analysis of these specimens is critically important in confirming the previous diagnosis, staging the extent of malignant disease, establishing whether or not the entire diseased area was removed (a process called "determination of the surgical margin", often using frozen section), identifying the presence of unsuspected concurrent diseases, and providing information for postoperative treatment, such as adjuvant chemotherapy in the case of cancer.In the determination of surgical margin of a surgical resection, one can use the bread loafing technique, or CCPDMA. A special type of CCPDMA is named after a general surgeon, or the Mohs surgery method.------6. Structure of microscope slideThe FSS had several facilities throughout the country, and provided scene-of-crime and forensic investigation services to police forces in England and Wales, as well as to the Crown Prosecution Service, HM Revenue and Customs, HM Coroners' Service, Ministry of Defence Police, British Transport Police and worldwide forensic services.When an executive agency, its two main headquarters were at 109 Lambeth Road (A3202), London and at Priory House on Gooch Street North in Birmingham.Its headquarters were close to the A452, near to where it crosses the M42. The Police in England and Wales spend 170million on forensic science.LaboratoriesIt had seven main laboratories across England and Wales:Trident Court, nr Solihull / BirminghamPriory House BirminghamHinchingbrooke Park, HuntingdonLondon (Lambeth) after becoming an executive agency as until then it had been the Met Lab.Audby Lane, Wetherby, LeedsUsk Road, ChepstowWashington Hall, ChorleyAldermaston Laboratory, Aldermaston------7. Subspecialties of microscope slideMany pathologists seek fellowship-level training, or otherwise pursue expertise in a focused area of surgical pathology. Subspecialization is particularly prevalent in the academic setting, where pathologists may specialise in an area of diagnostic surgical pathology that is relevant to their research, but is becoming increasingly prevalent in private practice as well. Subspecialization has a number of benefits, such as allowing for increased experience and skill at interpreting challenging cases, as well as development of a closer working relationship between the pathologist and clinicians within a subspecialty area. Commonly recognized subspecialties of surgical pathology include the following:Bone pathologyCardiac pathologyCytopathology (A board-certifiable subspecialty in the U.S.)Dermatopathology (A board-certifiable subspecialty in the U.S.)Endocrine pathologyGastrointestinal pathologyGenitourinary pathologyGynecologic pathologyHematopathology (A board-certifiable subspecialty in the U.S.)Neuropathology (A board-certifiable subspecialty in the U.S. and a recognised specialty in the U.K.)Ophthalmic pathologyPediatric pathology (A board-certifiable subspecialty in the U.S. and a recognised specialty in the U.K.)Pulmonary pathologyRenal pathologySoft tissue pathologyBreast pathology
Things You May Want to Know About Microscope Slide
Things You May Want to Know About Microscope Slide
An Introduction to microscope slideSurgical pathology is the most significant and time-consuming area of practice for most anatomical pathologists. Surgical pathology involves gross and microscopic examination of surgical specimens, as well as biopsies submitted by surgeons and non-surgeons such as general internists, medical subspecialists, dermatologists, and interventional radiologists.The practice of surgical pathology allows for definitive diagnosis of disease (or lack thereof) in any case where tissue is surgically removed from a patient. This is usually performed by a combination of gross (i.e., macroscopic) and histologic (i.e., microscopic) examination of the tissue, and may involve evaluations of molecular properties of the tissue by immunohistochemistry or other laboratory tests.Specimens of microscope slideThere are two major types of specimens submitted for surgical pathology analysis: biopsies and surgical resections.A biopsy is a small piece of tissue removed primarily for the purposes of surgical pathology analysis, most often in order to render a definitive diagnosis. Types of biopsies include core biopsies, which are obtained through the use of large-bore needles, sometimes under the guidance of radiological techniques such as ultrasound, CT scan, or magnetic resonance imaging. Core biopsies, which preserve tissue architecture, should not be confused with fine-needle aspiration specimens, which are analyzed using cytopathology techniques. Incisional biopsies are obtained through diagnostic surgical procedures that remove part of a suspicious lesion, whereas excisional biopsies remove the entire lesion and are similar to therapeutic surgical resections. Excisional biopsies of skin lesions and gastrointestinal polyps are very common. The pathologist's interpretation of a biopsy is critical to establishing the diagnosis of a benign or malignant tumor, and can differentiate between different types and grades of cancer, as well as determining the activity of specific molecular pathways in the tumor. This information is important for estimating the patient's prognosis and for choosing the best treatment to administer. Biopsies are also used to diagnose diseases other than cancer, including inflammatory, infectious, or idiopathic diseases of the skin and gastrointestinal tract, to name only a few.Surgical resection specimens are obtained by the therapeutic surgical removal of an entire diseased area or organ (and occasionally multiple organs). These procedures are often intended as definitive surgical treatment of a disease in which the diagnosis is already known or strongly suspected. However, pathological analysis of these specimens is critically important in confirming the previous diagnosis, staging the extent of malignant disease, establishing whether or not the entire diseased area was removed (a process called "determination of the surgical margin", often using frozen section), identifying the presence of unsuspected concurrent diseases, and providing information for postoperative treatment, such as adjuvant chemotherapy in the case of cancer.In the determination of surgical margin of a surgical resection, one can use the bread loafing technique, or CCPDMA. A special type of CCPDMA is named after a general surgeon, or the Mohs surgery method.Bibliography of microscope slideRichard Cote, Saul Suster, Lawrence Weiss, Noel Weidner (Editor). Modern Surgical Pathology (2 Volume Set). London: W B Saunders. ISBN0-7216-7253-1.CS1 maint: multiple names: authors list (link) CS1 maint: extra text: authors list (link).mw-parser-output cite.citationfont-style:inherit.mw-parser-output .citation qquotes:"""""""'""'".mw-parser-output .id-lock-free a,.mw-parser-output .citation .cs1-lock-free abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/6/65/Lock-green.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .id-lock-limited a,.mw-parser-output .id-lock-registration a,.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/d/d6/Lock-gray-alt-2.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .id-lock-subscription a,.mw-parser-output .citation .cs1-lock-subscription abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Lock-red-alt-2.svg/9px-Lock-red-alt-2.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/a/aa/Lock-red-alt-2.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registrationcolor:#555.mw-parser-output .cs1-subscription span,.mw-parser-output .cs1-registration spanborder-bottom:1px dotted;cursor:help.mw-parser-output .cs1-ws-icon abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Wikisource-logo.svg/12px-Wikisource-logo.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/4/4c/Wikisource-logo.svg");background-repeat:no-repeat;background-size:12px;background-position:right .1em center.mw-parser-output code.cs1-codecolor:inherit;background:inherit;border:inherit;padding:inherit.mw-parser-output .cs1-hidden-errordisplay:none;font-size:100%.mw-parser-output .cs1-visible-errorfont-size:100%.mw-parser-output .cs1-maintdisplay:none;color:#33aa33;margin-left:0.3em.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration,.mw-parser-output .cs1-formatfont-size:95%.mw-parser-output .cs1-kern-left,.mw-parser-output .cs1-kern-wl-leftpadding-left:0.2em.mw-parser-output .cs1-kern-right,.mw-parser-output .cs1-kern-wl-rightpadding-right:0.2em.mw-parser-output .citation .mw-selflinkfont-weight:inherit
Knowledge About Microscope Slide
Knowledge About Microscope Slide
1. History of microscope slideIn 1982, on the one-hundredth anniversary of Robert Koch's presentation, the International Union Against Tuberculosis and Lung Disease (IUATLD) proposed that 24 March be proclaimed an official World TB Day. This was part of a year-long centennial effort by the IUATLD and the World Health Organization under the theme "Defeat TB: Now and Forever. World TB Day was not officially recognized as an annual occurrence by WHO's World Health Assembly and the United Nations until over a decade later.In the fall of 1995, WHO and the Royal Netherlands Tuberculosis Foundation (KNCV) hosted the first World TB Day advocacy planning meeting in Den Haag, Netherlands; an event they would continue co-sponsor over the next few years. In 1996, WHO, KNCV, the IUATLD and other concerned organizations joined to conduct a wide range of World TB Day activities.For World TB Day 1997, WHO held a news conference in Berlin during which WHO Director-General Hiroshi Nakajima declared that "DOTS is the biggest health breakthrough of this decade, according to lives we will be able to save." WHO's Global TB Programme Director, Dr. Arata Kochi, promised that, "Today the situation of the global TB epidemic is about to change, because we have made a breakthrough. It is the breakthrough of health management systems that makes it possible to control TB not only in wealthy countries, but in all parts of the developing world, where 95 percent of all TB cases now exist."By 1998, nearly 200 organizations conducted public outreach activities on World TB Day. During its World TB Day 1998 news conference in London, WHO for the first time identified the top twenty-two countries with the world's highest TB burden. The next year, over 60 key TB advocates from 18 countries attended the three-day WHO/KNCV planning meeting for World TB Day 1999.U.S. President Bill Clinton marked World TB Day 2000 by administering the WHO-recommended Directly Observed Therapy, Short-Course (DOTS) treatment to patients at the Mahavir Hospital in Hyderabad, India. According to Clinton, "These are human tragedies, economic calamities, and far more than crises for you, they are crises for the world. The spread of disease is the one global problem for which . . . no nation is immune."In Canada, the National Collaborating Centre for Determinants of Health noted on World TB Day 2014 that 64% of TB cases reported nationally were among foreign-born individuals and 23% among Aboriginal people, highlighting TB as a key area of concern about health equity.Today the Stop TB Partnership, a network of organizations and countries fighting TB (the IUATLD is a member and WHO houses the Stop TB Partnership secretariat in Geneva), organizes the Day to highlight the scope of the disease and how to prevent and cure it.------2. Biography of microscope slideEarly lifeTweedy John Todd was born in 1789 in Berwick-Upon-Tweed where his father was a borough treasurer. Todd studied medicine at the University of Edinburgh, but did not complete his degree, joining the Royal Navy instead in 1809.Navy careerTodd began his career as a Royal Navy surgeon working in the Royal Naval Hospital at Plymouth. He later sailed to the East Indies and the Cape of Good Hope and was appointed surgeon on the flagship of Sir Robert Stopford aged only 23. While at the Cape of Good Hope aboard HMSLion, he performed experiments on the electric ray (Torpedo sp), which were published in the Philosophical Transactions of the Royal Society.Todd retired from the navy in 1816, and lived in Italy for 6 or 7 years. Following this he returned to his studies and completed two medical degrees, first at the University of Montpellier, then at the University of Aberdeen before ultimately settling in Brighton in 1829, where he developed a medical practice.Scientific WorkWhile studying and then working as a physician in Brighton, Todd continued to publish papers on natural history, including work on fireflies in "The Luminous Power of Some of the Lampyrides" in 1827. In 1831 wrote "The Book of Analysis. A New Method of Experience", in order to encourage physicians and scientists to apply the Baconian method of inductive reasoning to medicine and the other natural sciences.Todd began experiments on healing using newts at the British Naval base in Naples, and continued when he moved to Brighton. He conducted a series of experiments on the newts and other animals such as worms to study the processes of healing and regeneration of wounds and amputated body parts. These were the first experiments that proved that nerves have a role in healing, and that amphibian limbs cannot regenerate if the nerves are cut. He observed the healing process microscopically and collaborated with histologist Charles Ager to produce over 3,000 microscope slides to illustrate his findings. These slides are amongst the earliest surviving examples of the use of Canada balsam as a mountant.Todd's research was not well known in the second half of the 19th century, but was the basis of many studies in the early 20th century, and more recently the importance of Todd's work has been acknowledged by medical historians.Todd died from Tuberculosis on 4 August 1840, in Hurst, Sussex. He was aged 50 or 51. After his death his large collection of microscope slides came to the attention of Richard Owen, who was then the conservator of the Hunterian Museum at the Royal College of Surgeons in London, but would later become the first director of the Natural History Museum. Owen was impressed by the quality of the slides and in 1841 he purchased 1,500 of them for 150 to use in the teaching of surgeons. The slides are considered to be important in the history of medicine, histology and microscopy and they remain in the College's collection today.------3. Function of microscope slideBefore HM Government wind-up led by minister James Brokenshire, the FSS was the market leader in the supply of forensic science services to police forces in England and Wales, as well as being a source of training, consultancy and scientific support. The FSS originally set up and maintained the UK National DNA Database, but it is now run by the National Policing Improvement Agency (NPIA).The FSS suffered damage to its reputation following the failure to recover blood stains from a shoe in the murder of Damilola Taylor. Further damage occurred when the FSS failed to use the most up-to-date techniques for extracting DNA samples in cases between 2000 and 2005. This led the Association of Chief Police Officers (ACPO) to advise all police forces in England and Wales to review cases where samples had failed to give a DNA profile.TechnologiesThe FSS's innovative and highly sensitive DNA profiling technique called LCN (low copy number) was used in convicting Antoni Imiela (the M25 rapist) and Ronald Castree (for the murder of Lesley Molseed in 1975), but was questioned during the 2007 trial of a suspect in the Omagh bombing. However, a review by the CPS found that "the CPS has not seen anything to suggest that any current problems exist with LCN. Accordingly we conclude that LCN DNA analysis provided by the FSS should remain available as potentially admissible evidence". In addition, other Police Forces around the world are reviewing cases where LCN DNA profiling resulted in the successful prosecution of suspects. Included in this are several high-profile international cases including the murder of Swedish Foreign Minister Anna Lindh by Mijailo Mijailovic and in Australia, the murder of a backpacker Peter Falconio by Bradley John Murdoch and trial of Bradley Robert Edwards for the Claremont serial killings.In later years the FSS drew on internal expertise and key international experts to become a pioneer in forensic software and technology, notably DNA interpretation, databasing, and electronic forensics.------4. Bibliography of microscope slideRichard Cote, Saul Suster, Lawrence Weiss, Noel Weidner (Editor). Modern Surgical Pathology (2 Volume Set). London: W B Saunders. ISBN0-7216-7253-1.CS1 maint: multiple names: authors list (link) CS1 maint: extra text: authors list (link).mw-parser-output cite.citationfont-style:inherit.mw-parser-output .citation qquotes:"""""""'""'".mw-parser-output .id-lock-free a,.mw-parser-output .citation .cs1-lock-free abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/6/65/Lock-green.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .id-lock-limited a,.mw-parser-output .id-lock-registration a,.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/d/d6/Lock-gray-alt-2.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .id-lock-subscription a,.mw-parser-output .citation .cs1-lock-subscription abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/a/aa/Lock-red-alt-2.svg/9px-Lock-red-alt-2.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/a/aa/Lock-red-alt-2.svg");background-repeat:no-repeat;background-size:9px;background-position:right .1em center.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registrationcolor:#555.mw-parser-output .cs1-subscription span,.mw-parser-output .cs1-registration spanborder-bottom:1px dotted;cursor:help.mw-parser-output .cs1-ws-icon abackground-image:url("//upload.wikimedia.org/wikipedia/commons/thumb/4/4c/Wikisource-logo.svg/12px-Wikisource-logo.svg.png");background-image:linear-gradient(transparent,transparent),url("//upload.wikimedia.org/wikipedia/commons/4/4c/Wikisource-logo.svg");background-repeat:no-repeat;background-size:12px;background-position:right .1em center.mw-parser-output code.cs1-codecolor:inherit;background:inherit;border:inherit;padding:inherit.mw-parser-output .cs1-hidden-errordisplay:none;font-size:100%.mw-parser-output .cs1-visible-errorfont-size:100%.mw-parser-output .cs1-maintdisplay:none;color:#33aa33;margin-left:0.3em.mw-parser-output .cs1-subscription,.mw-parser-output .cs1-registration,.mw-parser-output .cs1-formatfont-size:95%.mw-parser-output .cs1-kern-left,.mw-parser-output .cs1-kern-wl-leftpadding-left:0.2em.mw-parser-output .cs1-kern-right,.mw-parser-output .cs1-kern-wl-rightpadding-right:0.2em.mw-parser-output .citation .mw-selflinkfont-weight:inherit------5. Specimens of microscope slideThere are two major types of specimens submitted for surgical pathology analysis: biopsies and surgical resections.A biopsy is a small piece of tissue removed primarily for the purposes of surgical pathology analysis, most often in order to render a definitive diagnosis. Types of biopsies include core biopsies, which are obtained through the use of large-bore needles, sometimes under the guidance of radiological techniques such as ultrasound, CT scan, or magnetic resonance imaging. Core biopsies, which preserve tissue architecture, should not be confused with fine-needle aspiration specimens, which are analyzed using cytopathology techniques. Incisional biopsies are obtained through diagnostic surgical procedures that remove part of a suspicious lesion, whereas excisional biopsies remove the entire lesion and are similar to therapeutic surgical resections. Excisional biopsies of skin lesions and gastrointestinal polyps are very common. The pathologist's interpretation of a biopsy is critical to establishing the diagnosis of a benign or malignant tumor, and can differentiate between different types and grades of cancer, as well as determining the activity of specific molecular pathways in the tumor. This information is important for estimating the patient's prognosis and for choosing the best treatment to administer. Biopsies are also used to diagnose diseases other than cancer, including inflammatory, infectious, or idiopathic diseases of the skin and gastrointestinal tract, to name only a few.Surgical resection specimens are obtained by the therapeutic surgical removal of an entire diseased area or organ (and occasionally multiple organs). These procedures are often intended as definitive surgical treatment of a disease in which the diagnosis is already known or strongly suspected. However, pathological analysis of these specimens is critically important in confirming the previous diagnosis, staging the extent of malignant disease, establishing whether or not the entire diseased area was removed (a process called "determination of the surgical margin", often using frozen section), identifying the presence of unsuspected concurrent diseases, and providing information for postoperative treatment, such as adjuvant chemotherapy in the case of cancer.In the determination of surgical margin of a surgical resection, one can use the bread loafing technique, or CCPDMA. A special type of CCPDMA is named after a general surgeon, or the Mohs surgery method.
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